Now accepting early access partners

Small RNA Analysis,
Automated.

Upload FASTQs. Get differential expression, novel transcripts, and disease annotations from curated databases — all in one platform.

Request Early Access See How It Works
Built by RNA scientists Human GRCh38 Cloud & on-premise
0 QC Metrics per Run
0 Steps to Results
0 Unified Interface
The Problem

Small RNA Analysis Has a Structural Gap

Analysis Is a Bottleneck

Most teams wait weeks for results from overloaded collaborators or outsourced bioinformatics.

Fragmented Toolchains

Stitching together Trim Galore, Bowtie, miRDeep2, and DESeq2 demands deep expertise and constant maintenance.

Results Without Context

Standard pipelines stop at differential expression. You still cross-reference disease databases manually.

The Difference

Manual Pipeline vs. Autoligo

Without Autoligo

  • ✕Weeks of turnaround waiting on collaborators
  • ✕5+ tools to install, configure, and maintain
  • ✕Results stop at a count matrix
  • ✕Manual database lookups for every miRNA
  • ✕Hard to reproduce across labs and runs

With Autoligo

  • ✓Upload FASTQs, results ready in minutes
  • ✓One platform — no setup, no maintenance
  • ✓Differential expression, QC, novel transcripts
  • ✓Disease annotations delivered automatically
  • ✓Fully parameterized, versioned, reproducible
How It Works

Four Steps from Upload to Insight

Autoligo handles the full analytical chain so you can focus on the science.

STEP 01

Upload FASTQs

Drag and drop raw sequencing files. Format and metadata are validated automatically.

STEP 02

Run the Pipeline

QC, trimming, alignment, quantification, differential expression, and novel transcript prediction.

STEP 03

Explore Results

Volcano plots, QC dashboards, expression heatmaps, and per-miRNA annotation panels.

STEP 04

Annotated Results

Every miRNA cross-referenced against curated disease and target databases. Data to hypothesis, one interface.

What You Get

Publication-Ready Results, Built In

Every run delivers a complete analytical package — no assembly required.

QC Report
Total Reads
92%
Alignment
87%
miRNA Mapped
78%
Adapter Trim
99%
Duplication
12%
Differential Expression
log₂ Fold Change
Significant Upregulated NS
Annotation Panel
miRNA log₂FC Status
hsa-miR-21-5p +2.4 DE Oncology
hsa-miR-155-5p +1.8 DE Immune
hsa-miR-126-3p −1.6 DE Cardiac
hsa-miR-223-3p +1.2 DE
Platform Capabilities

Everything a smRNA-Seq Study Requires

Novel Transcript Prediction

De novo miRNA candidate discovery via miRDeep2, embedded in every run. Capture signals beyond known miRBase annotations automatically.

Differentiator

Reproducible by Default

Every run is fully parameterized and logged. Complete audit trail from FASTQ to results.

Core

Differential Expression

Define contrasts, set FDR thresholds, and get annotated results with interactive visualizations.

Core

35+ QC Metrics per Run

Read quality, adapter content, alignment rates, and library complexity. Exportable for publication.

Transparency

Cloud or On-Premise

Deploy as cloud-hosted or on-premise for data sovereignty. Same interface, either environment.

Enterprise
Who It's For

Built for the Teams Doing the Science

Biotech & Pharma

RNA Biology Teams Without Dedicated Bioinformatics

  • Eliminate analysis bottlenecks
  • Audit-ready outputs for submissions
  • Accelerate target prioritization
CROs & Service Labs

Upgrade Your smRNA-Seq Deliverable

  • Higher-value deliverables, no extra headcount
  • Consistent infrastructure across projects
  • On-premise for sensitive client data
Academic Labs

From PI Labs to Core Facilities

  • No bioinformatics FTE required
  • Grant-ready QC documentation
  • Per-project access and sharing
Bioinformaticians

Automate the Routine, Focus on the Novel

  • Parameterized, auditable runs
  • Configurable annotation databases
  • API access (coming soon)
Configurable Annotation Stack
Disease Associations
Target Gene Networks
Pathway Enrichment
Tissue Expression & Clinical Context
Your Custom Databases
Annotation Layer

A Configurable Annotation Layer

When your analysis completes, every detected miRNA is annotated against comprehensive disease and target knowledge bases. Swap databases, add proprietary sources, or extend with program-specific annotations. Academic licensing available.

  • Disease associations surfaced alongside differential expression results
  • Target gene networks linked to experimental expression changes
  • Configurable sources — add proprietary or program-specific annotations

See Autoligo With Your Own Data

We are onboarding early access partners now. We’ll review your request and follow up by email.

Prefer email? Reach us at [email protected]